Cell-Based Efficacy Testing Platform:

• Inflammation: Mouse macrophage RAW 264.7 cells are induced by LPS to measure Nitric Oxide (NO).
• Arthritis: Human chondrocyte SW1353 or human synovial cell SW982 are induced by IL-1β, followed by measurement of NO (Nitric Oxide).
• Bone Health:
  (1) Osteoclast assay: Mouse macrophage RAW 264.7 cells are differentiated into osteoclasts, and enzyme activity (TRAP activity) is measured.
  (2) Osteoblast assay: Mouse pre-osteoblast MC3T3-E1 cells are differentiated into mature osteoblasts, and enzyme activity (AP assay) and calcium deposition are measured.
• Lipid Droplet Accumulation: Mouse preadipocyte 3T3-L1 and human hepatocellular carcinoma cells HepG2 are induced to form lipid droplets. Qualitative and quantitative analysis is conducted using Nile Red or Oil Red, along with triglyceride quantification.
• Liver Fibrosis: TGF-β1 is used to induce LX-2 cells, and the expression of COL1A1, COL1A2, COL3A1, COL4A1, and MMP9 is measured.
• Sarcopenia: Mouse myoblast C2C12 cells are differentiated into myotubes, followed by Dexamethasone-induced myotube atrophy, and the width of fused cells is measured.
• Neuroprotection: Rat adrenal pheochromocytoma PC12 cells are differentiated and then damaged by Corticosterone to evaluate cell protective effects.
• Wound Healing: A circular gap is created using Stoppers or Inserts to evaluate the migration of human keratinocyte HaCaT cells.
• Whitening: Mouse melanoma B16-F10 cells are induced by α-MSH, and melanin deposition is measured.
• Anti-wrinkle: Human fibroblast HS68 cells are irradiated with UVA, and the expression of pro-collagen 1A1 is measured.
• DPPH Antioxidant Assay (Chemical Reaction)
• Hair Follicle Proliferation: Human dermal papilla cells are used to evaluate cell proliferation effects.